-aspartate receptor activity in hippocampal CA3 circuits

lthough Zn 2 is contained in large amounts in the synaptic terminals of hippocampal mossy fibers (MFs), its physiological role in synaptic transmission is poorly understood. By using the newly developed high-sensitivity Zn 2 indicator ZnAF-2, the spatiotemporal dynamics of Zn 2 was monitored in rat hippocampal slices. When high-frequency stimulation was delivered to the MFs, the concentration of extracellular Zn 2 was immediately elevated in the stratum lucidum, followed by a mild increase in the stratum radiatum adjacent to the stratum lucidum, but not in the distal area of stratum radiatum. The Zn 2 increase was insensitive to a non– N -methylD -aspartate (NMDA) receptor antagonist but was A efficiently attenuated by tetrodotoxin or Ca 2 -free medium, suggesting that Zn 2 is released by MF synaptic terminals in an activity-dependent manner, and thereafter diffuses extracellularly into the neighboring stratum radiatum. Electrophysiological analyses revealed that NMDA receptor– mediated synaptic responses in CA3 proximal stratum radiatum were inhibited in the immediate aftermath of MF activation and that this inhibition was no longer observed in the presence of a Zn 2 -chelating agent. Thus, Zn 2 serves as a spatiotemporal mediator in imprinting the history of MF activity in contiguous hippocampal networks. We predict herein a novel form of metaplasticity, i.e., an experiencedependent non-Hebbian modulation of synaptic plasticity.